;������s:��uO:kj77�f��$+��X�2o�囲�^��2�.w����P� ���K�d�K��|��DO��+[�t�E�է�`��B���A��ve��H To place an order via phone, email or for requesting a quote, please provide the product’s name, number and catalog number. Ampliqon TEMPase Hot Start DNA Polymerase 2x Master Mix is a ready to use master mix composed of TEMPase Hot Start DNA Polymerase, dNTPs, MgCl 2 and either TEMPase Buffer C (a balanced KCI/(NH 4) 2 SO 4 Tris buffer system) or TEMPase Buffer A (a (NH 4) 2 SO 4 tris buffer system).. Prevent extension of non-specifically bound primers; Simple, convenient workflow. Description. Different hot-start enzymes were employed: HotStarTaq DNA Polymerase from QIAGEN (, HotStarTaq DNA Polymerase is supplied with a streamlined, optimized protocol for fast and easy PCR setup. Substrate analogs: dNTP, ddNTP, dUTP, biotin-11-dUTP, DIG-11-dUTP, fluorescent-dNTP/ddNTP �0 �mMӗ4Q�"�u Reactions can be set up at room temperature, ensuring greater convenience and ease of use (see figure ", Addressing critical factors and new solutions, HotStarTaq DNA Polymerase; HotStarTaq Master Mix Kit - For highly specific hot-start PCR without optimization. Each lot of HotStarTaq DNA Polymerase is subjected to a comprehensive range of quality control tests, including a stringent PCR specificity and reproducibility assay in which low-copy targets are amplified. Unlike other commonly used PCR additives such as DMSO, Q-Solution is used at just one working concentration, is nontoxic, and PCR purity is guaranteed. HotStarTaq DNA Polymerase, a modified form of Taq DNA Polymerase, provides high specificity in hot-start PCR. The inhibitor binds reversibly to the enzyme, inhibiting polymerase activity at temperatures below 45°C, but releases the enzyme during normal cycling conditions, allowing reactions to be set up at room temperature. Have you tested the effect of inhibitors on PCR performance? A thermostable inhibitor (patent-pending) of the Taq DNA Polymerase is released at high temperatures, thereby immediately activating the enzyme. Hot Start Taq DNA Polymerase. The hot start PCR is the most advanced modification of conventional PCR in which one of the PCR reagents is activated only after heating (in PCR). This step heats the solutions to 94-98°C for DNA polymerase activation. Hot-Start PCR is a variant of PCR commonly employed to prevent the amplification of the non-target sequence. %PDF-1.6 %���� One Taq Hot Start DNA Polymerase is supplied with two 5X buffers: (Standard and GC), as well as a High GC Enhancer solution. Can Taq DNA Polymerase use RNA as a template, and generate false positives in "no-RT" controls? Can QIAGEN's Taq- and HotstarTaq DNA Polymerases be used for cycle sequencing? Non-specific binding often leads to primer dimers and mis-primed/false primed targets. Successful PCR requires a number of components, including a DNA polymerase capable of tolerating high temperature incubations (94°C or higher) that occur during a typical thermal cycling protocol. In Hot Start activation, primer extension is blocked until the reaction mixture reaches an elevated, Hot Start temperature, where the stringency of the primer/target hybridization is optimal for specificity, and primer complexes are dissociated. Do CoralLoad dyes supplied in various QIAGEN PCR Kits interfere with downstream applications? What should the starting template DNA quality and quantity be for PCR? This is only essential for Hot-start PCR. Hot-Start DNA Polymerases & Master Mixes—Thermo Scientific Hot-start PCR prevents the amplification of non-specific products, amplifies low abundance targets and offers convenient room temperature reaction setup. Once this temperature has been reached, the inhibitor releases the enzyme. 4[�!�j�����pE�n!˰Z����ę���X���j�d����p��k?����p��������V��w~n�������i��&~&�}���S_P��ô�֎4ܿ_�u����;��������5Nl>q��9ʼn�%Nd�X�D��ߢ��% Q-Solution, an innovative PCR additive that facilitates amplification of difficult templates by modifying the melting behavior of DNA, is also provided with HotStarTaq DNA Polymerase. endstream endobj 166 0 obj <>stream The newer ones use antibodies which require less activation and the really new ones use aptamers which require activation steps of around 30 seconds. Hot start PCR is a method which prevents DNA polymerase extension at lower temperature to prevent non-specific binding to minimise yield loss. The polymerase combines the high specificity, sensitivity, and minimal optimization of HotStarTaq DNA Polymerase, with a fast 5-minute activation time. What makes QIAGEN's 10x Taq and HotStarTaq DNA Polymerase PCR buffer superior? We offer different hot-start DNA polymerases to support your everyday research needs. HotStarTaq DNA Polymerase is activated by a 15-minute, 95°C incubation step, which can easily be incorporated into existing thermal cycling programs. hޤTmk�0�+��22�Y� %��&����u ��������V����S�4a�6��{��sϙF��D��D�d@4.�� �3EM&�q����ﳫ+z��!vԯl�H��$��:�U���$o�\? Documents iTaq™ DNA polymerase is an antibody-mediated hot-start DNA polymerase suitable for both PCR and real-time PCR applications. The polymerase chain reaction (PCR) is a widely used technique, and the foundation of numerous diagnostic applications that seek to detect minute amounts of DNA via exponential amplification. the antibody-mediated hot-start employed by iTaq Polymerase sequesters Taq activity prior to the initial PCR denaturation step. iTaq DNA polymerase is highly specific, sensitive, and easy to use. Hot Start DNA polymerase control is achieved by chemical or antibody modification of the enzyme. Hot Start Taq DNA Polymerase is a mixture of Taq DNA Polymerase and an aptamer-based inhibitor. biotechrabbit Hot-start PCR products include highly purified YourTaq™ Hot Start DNA Polymerase which is optimized for high yield of amplification of 0.1–3 kb DNA targets, even from low copy number. Product Information. Fig. Assay for polymerase activity prior to thermal activation. DNA Polymerase that requires a pre-activation step at 95°C for 5 minutes in order to achieve a fully functional polymerase enzyme. 1: �(d5�aA�m2��dd�i��b�1�v��&�M0 t�?6 endstream endobj 167 0 obj <>stream “ Hot start PCR = One of the components starts its activity under the hot condition of PCR.” iTaq DNA Polymerase is suitable for many PCR applications. The enzyme aptamer-oligonucleotide mixture is a reversible, temperature-dependent hot start system. 66����y%@��wfJq4H�@!� H��T��$�����U[��C�I�Fe�� �%�2��md What is the composition of the QIAGEN 10x PCR Buffer in Taq- and HotStarTaq DNA Polymerase Kits? Hot Start PCR is a technique that inhibits Hot Start Taq polymerase activity or the incorporation of modified dNTPs during reaction set up until a heat activation step occurs. Chemically modified hot start enzymes require up to 10 minutes activation whereas antibody mediated hot start enzymes are activated within 1 minute. Hot Start Taq, Sample Pack. HotStarTaq DNA Polymerase is suitable for a wide variety of applications, including challenging applications, such as amplification of: You are not authorized to download the resource, For highly specific amplification with minimal optimization. The aptamer allows a reversible and immediate activation of the polymerase, leading to specific priming and a very fast PCR. HotStarTaq DNA Polymerase uses a chemically mediated hot start that, unlike, antibody-mediated systems, leads to complete inactivation of the polymerase until the initial heat activation step at the start of … This product is not intended for the diagnosis, prevention, or treatment of a disease. HotStarTaq DNA Polymerase is supplied with the unique QIAGEN PCR Buffer, which minimizes nonspecific amplification products, primer dimers, and background. Benefits of EagleTaq DNA Polymerase: Maximize PCR specificity, sensitivity, and target yield. 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